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Table 6 Sensitivity, specificity and agreement of the different diagnostic tests in Thailand

From: Development, validation and evaluation of a rapid PCR-nucleic acid lateral flow immuno-assay for the detection of Plasmodium and the differentiation between Plasmodium falciparum and Plasmodium vivax

Detection line Sensitivity (95% CI) Specificity (95% CI) Positive Predictive Value (95% CI) Negative Predictive Value (95% CI) Kappa
Overall pan-Plasmodium
SD-Bioline vs Microscopy (n = 377) 0.813 (0.723-0.879) 0.985 (0.960-0.995) 0.956 (0.884-0.986) 0.930 (0.892-0.956) 0.83
Microscopy vs PCR-NALFIA (n = 381) 0.869 (0.787-0.924) 0.949 (0.914-0.970) 0.869 (0.787-0.924) 0.949 (0.914-0.971) 0.82
SD-Bioline vs PCR-NALFIA (n = 377) 0.822 (0.734-0.887) 0.989(0.965-0.997) 0.967 (0.900-0.991) 0.934(0.897-0.958) 0.85
P. falciparum
SD-Bioline vs Microscopy (n = 377) 1 (0.874-1) 0.985 (0.964-0.994) 0.872 (0.718-0.952) 1 (0.986-1) 0.92
Microscopy vs PCR-NALFIA (n = 381) 0.773 (0.617- 0.880) 1 (0.986-1) 1 (0.874-1) 0.971 (0.946-985) 0.88
SD-Bioline vs PCR-NALFIA (n = 377) 0.796 (0.642-0.897) 0.988 (0.967-0.996) 0.898 (0.748-0.967) 0.973 (0.948-0.987) 0.84
P. vivax*
SD-Bioline vs Microscopy (n = 338) 0.722 (0.602-0.818) 1 (0.982-1) 1 (0.914-1) 0.930 (0.892-0.956) 0.78
Microscopy vs PCR-NALFIA (n = 381) 0.845 (0.735-0.916) 0.951 (0.920-0.972) 0.80 (0.689-0.880) 0.964 (0.935-0.981) 0.78
SD-Bioline vs PCR-NALFIA (n = 338) 0.754 (0.633-0.846) 1 (0.982-1) 1 (0.914-1) 0.941 (0.905-0.964) 0.82
  1. *Because the pLDH line on the SD-bioline RDT also detects P. falciparum, all P. falciparum samples were excluded from the analysis with SD-Bioline.