Figure 2

A (arrow and Ai insert) and B: HES-stained bone marrow sections showing immature gametocytes - elongated shape, pink and purple staining, finely distributed pigment – outside small vessels (vl) and in close contact with resident cells such as adipocytes (ad) or erythroblasts (e). The dotted line in A highlights the vessel wall. C: Mature stage V gametocyte close to a mature red blood cell (C, r) in the lumen of a vessel. D and E: Immunohistochemistry (antibodies revealed with peroxydase) showing gametocytes (arrows) distant from CD34-positive vascular structures (vl) or CD68-positive macrophages (mf).