shows the results of mouse CM 5-a hearing impaired animal-for cleaved caspase 3 immunohistochemistry. A positive cleaved caspase 3 reaction is brown. a) shows an overview of the cochlea duct in the middle turn. The brown staining in the fibrocytes type 1 of the spiral ligament (FC1), the spiral ganglion cells (SGC) and the limbus (Li) indicates positive labelling for cleaved caspase 3. (magnification 200×). The brown staining in the stria vascularis is a physiologic melanin (M) pigmentation. b) shows the organ of Corti. The inner hair cells (IHC) and the outer hair cells (OHC) are well preserved. No immunohistochemical labelling can be visualized. c) shows a close up of the spiral ganglion cells (SGC) with a positive immunohistochemical labelling for cleaved caspase 3. d) visualizes the positive cleaved caspase 3 reaction in the fibrocytes type 1 of the spiral ligament.