shows the Evans blue evaluation. a) demonstrates fluorescence microscopic evaluation of mouse Evans CM 4. The red colour signals extravasation of the Evans blue in the tissue, indicating a disturbance of the blood labyrinth barrier. A strong staining can be detected in the stria vascularis (SV) and the limbus (Li). Furthermore positive staining can be detected in the spiral ligament (SL). b) shows the fluorescence microscopic result for the control mouse Evans control. No extravasion of Evans blue can be detected. The shutter time with 231 ms is equal to Figure 4a. c) shows the gross morphology of the brain of the mouse Evans CM 4. The blue colour indicates a breakdown of the blood brain barrier. d) shows the gross morphology of the brain of the mouse Evans Control. No extravasation of Evans blue is visible, indicating a proper function of the blood brain barrier.