COPAS profile of the homozygous DSX strain and sexual selection. A. About 5,000 freshly hatched larvae of the homozygous DSX line were subjected to COPAS analysis and sorting. The data generated by COPAS was treated with WinMDI software to analyse the results and to express results as artificially colored regions corresponding to males (red) and to females (green) on the fluorescence diagram (on the right). Dot colours are the same as in the time-of-flight vs extinction diagram (left). B. A total of 2,000 larvae of each sex were selected with COPAS and grown to adulthood. Careful visual examination of the cages did not reveal the presence of any adult of the wrong sex. Left panels: male mosquitoes, as identified by the hairy antennae; right panels: female mosquitoes. Bottom panels are close-ups of top panels.