Table 1 Primers and probes used for sequencing and TqPCR quantification
Type | Primer/Probe | 5’ Fluorophore | Sequence | 3’ Quencher |
|---|---|---|---|---|
sp | Pf B | GGCAAATAACTTTATCATAGAATTGAC | ||
sp | Pf F | TTTATGTATTGGTATAACATTCGG | ||
sp | Pv1087f | GTGGCCGCCTTTTTGCT | ||
sp | Pv1196r | CCTCCCTGAAACAAGTCATCG | ||
sp | PovS | CCAAGCCCAGATAATAAGGAAGGT | ||
sp | PovR | TTCGTGCACTTCAACTTACATTCAGT | ||
sp | PmalS | GGAGGAATGGTCACCATGTAGTGT | ||
sp | PmalA | CAAATTTCAGTTTCAAGGTCACTTAA | ||
sp | pPf | FAM | TACACTACCAACACATGGGGCTACAAGAGGT | BHQ1 |
sp | pPv | HEX | CATCTACGTGGACAACGGGCTCAACA | BHQ1 |
sp | pPo | FAM | TTATTGTCCTCTGGGTTTGGAACTTTGCC | BHQ1 |
sp | pPmal | HEX | ATTTTTTGCATCAACCTTTCTTCTAGCCC | BHQ1 |
C/W | POF | ATAAACTATGCCGACTAGGTT | ||
C/W | POR | ACTTTGATTTCTCATAAGGTACT | ||
C/W | pPOW | HEX | AATTCCTTTTGGAAATTTCTTAGATTG | BHQ1 |
C/W | pPOC | FAM | TTCCTTTCGGGGAAATTTCTTAGA | BHQ1 |
seq | P1F-Up | TCCATTAATCAAGAACGAAAGTTAAG | ||
seq | 18S R | TAATGATCCTTCCGCAGGTTCACC | ||
seq | LDH ov D21 | GTTCTCGTTGGTCAGGAATGATA | ||
seq | LDH ov C915 | GGCATCATCAAACATCTTCTTTTCT |