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Table 2 Genotype determination by sequencing and POCPOW assay

From: Real-time PCR assay for discrimination of Plasmodium ovale curtisi and Plasmodium ovale wallikeri in the Ivory Coast and in the Comoros Islands

   

TqPCR

 

Gene

Type

Sample (n = 70)

POC (n = 44)

POW (n = 26)

% similarity

SSU rRNA

curtisi

14

14

0

100

SSU rRNA

wallikeri

9

0

9

100

LDH

curtisi

30

30

0

100

LDH

wallikeri

17

0

17

100

  1. Samples were first identified by amplification of the SSU rRNA and ldh sequences, thanks to the primers displayed below. The results were compared with the TqPCR results and the similarity was calculated. Four P. o. curtisi samples and four P. o. wallikeri samples were sequenced for both SSU rRNA and ldh genes.