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Table 1 Cytometric analysis of Plasmodium falciparum GFP + gametocytes (line 164/GFP) before and after sorting

From: Optimization of flow cytometric detection and cell sorting of transgenic Plasmodium parasites using interchangeable optical filters

Cell population % GFP+ cells-Exp1 % GFP+ cells-Exp2 % GFP+ cells-Exp3
Before sorting 0.05% 0.06% 0.03%
After sorting 95.1% 94.7% 93.1%
  1. Gametocytes were collected in an equal volume of PBS supplemented with 1% BSA, HEPES and L-glutamine, subsequently subjected to imaging cytometer analysis and quantified events were reported. Data are percentage of GFP+ gametocytes (from line 164/GFP) acquired with flow cytometer FACSAria (before sorting) and with imaging cytometer Imagestream 100 (after sorting).