Optimization of flow cytometric detection and cell sorting of transgenic Plasmodium parasites using interchangeable optical filters

Table 1 Cytometric analysis of Plasmodium falciparum GFP ⁺ gametocytes (line 164/GFP) before and after sorting

Cell population	% GFP⁺ cells-Exp1	% GFP⁺ cells-Exp2	% GFP⁺ cells-Exp3
Before sorting	0.05%	0.06%	0.03%
After sorting	95.1%	94.7%	93.1%

Gametocytes were collected in an equal volume of PBS supplemented with 1% BSA, HEPES and L-glutamine, subsequently subjected to imaging cytometer analysis and quantified events were reported. Data are percentage of GFP⁺ gametocytes (from line 164/GFP) acquired with flow cytometer FACSAria (before sorting) and with imaging cytometer Imagestream 100 (after sorting).

ISSN: 1475-2875