Figure 9
Cellular immune responses induced by the various regimens are strain-specific. Fine specificity of T cell responses induced in ICR mice (Panel A, B) by various immunization regimens. IFN-γ responses (Panel A) and IL-4 (Panel B) responses were measured after ex vivo stimulations with MSP142 and subunit fragments (antigen dose. for ex vivo stimulation = 5 μg/mL) by ELISpot analysis. Data are expressed as the mean (+/− SEM) number of cytokine spot producing cells (SFC) per 106 splenocytes. Data are from two independent immunization experiments (n = 20/group). Cells from mice immunized only with saline/Montanide ISA-720 and splenocytes from the various immunization regimens stimulated with GST as control antigen did not yield responses above medium control.