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Table 2 PCR programs for PfATP6 codons 263, 623 and 769

From: Status of potential Pf ATP6 molecular markers for artemisinin resistance in Suriname

PfATP6 Codon

PCR

Temp.

Time

N. of cycles

Primers

263

1 st round PCR

92 °C

3 min

1 cycle

263P1 and 263P2

92 °C

30 sec

30 cycles

47 °C

45 sec

65 °C

1 min

68 °C

5 min

1 cycle

4 °C

Hold

1 cycle

2 nd round PCR

95 °C

3 min

1 cycle

263WT and 263 N1* 263 M and 263 N1

95 °C

45 sec

20 cycles

62 °C

1 min

72 °C

1 min

72 °C

10 min

1 cycle

4 °C

Hold

1 cycle

623-769

1 st round PCR

94 °C

5 min

1 cycle

ATP1 and ATP2

94 °C

30 sec

45 cycles

44 °C

45 sec

68 °C

1 min

68 °C

5 min

1 cycle

4 °C

Hold

1 cycle

623

2 nd round PCR

94 °C

5 min

1 cycle

623WT and 623 N2* 623 M and 623 N2

94 °C

30 sec

20 cycles

40 °C

45 sec

68 °C

1 min

70 °C

5 min

1 cycle

4 °C

Hold

1 cycle

769

2 nd round PCR

94 °C

5 min

1 cycle

769 N1 and 769 N2

94 °C

30 sec

25 cycles

56 °C

45 sec

68 °C

1 min

68 °C

5 min

1 cycle

4 °C

Hold

1 cycle

  1. (*) Second round PCR for codon 263 and 623 were performed in single reactions with either the wild type (WT) or mutant (M) primer.
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Malaria Journal

ISSN: 1475-2875

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