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Figure 5 | Malaria Journal

Figure 5

From: Neutral lipids associated with haemozoin mediate efficient and rapid β-haematin formation at physiological pH, temperature and ionic composition

Figure 5

MPG exhibited fast kinetics at 2.15 L/H (■) and 1.07 L/H mol ratio (▲) with k= 0.61 ± 0.07 and 0.40 ± 0.03 min−1respectively, while at 0.54 L/H mol ratio (), the reaction is much slower with k= 0.085 ± 0.006 min−1, similar to that of NLB (A). The reaction with MSG at the highest lipid concentration (2.15 L/H) is slower than NLB and MPG at 0.54 L/H mol ratio, with k = 0.044 ± 0.007 min−1 (B). Kinetics with DLG at 1.07 L/H (▲) and 0.54 L/H () appear to be faster than that of NLB at same mol ratio with k = 1.2 ± 0.2 and 0.27 ± 0.02 min−1 respectively, while at 0.27 L/H mol ratio (□) the reaction was slower with k = 0.058 ± 0.009 min−1 (C). DOG mediated exceptionally fast kinetics at 1.07 L/H (▲) and 0.54 L/H mol ratio () with k = 2.4 ± 0.2 and 0.69 ± 0.08 min−1 respectively, while the kinetics at 0.27 L/H (□) were slower with k = 0.094 ± 0.008 min−1, comparable to NLB and MPG at the mol ratio of 0.54 L/H (D). Citric buffer (50 mM, pH 4.8), 37°C. Constant total Fe(III)PPIX concentration of 30 μM. Error bars represent SEM (n = 4, except D for which n = 3).

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