Skip to main content

Table 1 Effects of biologically relevant cations and anions on kinetics of β-haematin formation mediated by MPG (0.54 L/H), citric buffer (50 mM, pH 4.8), 37°C a

From: Neutral lipids associated with haemozoin mediate efficient and rapid β-haematin formation at physiological pH, temperature and ionic composition

Added ion or biomolecule Concentration (mM) k(min−1) ± SEM (n = 4)
  (control) 0.085 ± 0.006
Na+ 8 RBC concentration 0.067 ± 0.006
Na+ 150 serum concentration 0.031 ± 0.004
K+ 150 RBC concentration 0.045 ± 0.005
Mg2+ 1.75 RBC concentration 0.038 ± 0.005f
Mg2+ 1.5 serum concentration 0.050 ± 0.005
Ca2+ 3 serum concentration 0.030 ± 0.005f
H2PO4 1 RBC concentrationb 0.033 ± 0.004
HCO3 15 RBC concentrationc 0.026 ± 0.004
ATP 1.5 RBC concentration 0.039 ± 0.005
all ions combined d RBC concentrations 0.037 ± 0.006
all ions combined e serum concentrations 0.037 ± 0.003
glutathione 2.48 RBC concentration 0.024 ± 0.003g
  1. aAll ions were added to 50 mM citrate/sodium citrate buffer, which represents a background value. bTotal RBC phosphates. cAt pH 4.8 present as CO2. d 8 mM Na+, 150 mM K+, 1 mM H2PO4, 15 mM HCO3 and 1.5 mM ATP. e 150 mM Na+, 1.75 mM Mg2+ and 3 mM Ca2+. f 50 mM acetate/acetic acid buffer instead of citrate. g Under Ar, all solutions purged with Ar.