Effect of sample dilution in pLDH detection – ELISA standardization. Vertical bars represent mean optical density (OD) values ± SD obtained from PbAGFP cultured schizonts at 0.5% or 1 % of parasitaemia and uninfected red blood cells (unRBC) at 1% of haematocrit. Grey and white bars represent schizonts culture at 1% and 0.5% of parasitaemia, respectively. Three different concentrations of 19G7 (secondary antibody) were tested (X-axis). Mean is derived from three independent assays.