Flow cytometry analyses of synchronized infections of P. berghei in C57BL/6 mice. A) Panel showing uninfected red blood cells (negative controls). B) Asexual blood-stages of PbAGFP from infected mice with normal day-night light regime. C) Highly fluorescent mature schizonts purified after 22 hours of in vitro culture used to generate synchronized P. berghei infections in mice. D) Rings, E) trophozoites and F) schizont-enriched populations from synchronized infections after one cycle of replication in mice. *hpi=hours post-infection.