Figure 1From: A flow cytometry-based workflow for detection and quantification of anti-plasmodial antibodies in vaccinated and naturally exposed individualsRepresentative overlay showing the anti-plasmodial Ab responses of a semi-immune individual vaccinated with GMZ2. The best separation between the negative and positive fluorescent cells is obtained when serum was diluted at 1/4,000. Test and control samples were treated as described in the methods. Note the increase in fluorescence intensity as shown by the shift to the right when parasites were incubated with serum diluted 1/32,000 (blue curve), 1/16,000 (orange curve), 1/8,000 (light green curve), 1/4,000 (green curve) or the control (red line) and the overall higher response after vaccination (Day 84).Back to article page