
Manual gating


Gabonese adults (n = 37)^{a}

MFI day 0

MFI day 84

PPFC day 0

PPFC day 84


kmeans

r = 0.95

r = 0.89

ρ = 0.04^{§}

ρ = 0.14^{§}


r^{2} = 0.91

r^{2} = 0.79
  
EM*

r = 0.92

r = 0.89

ρ = 0.89

ρ = 0.94


r^{2} = 0.85

r^{2} = 0.80
  
Overlap subtraction

r = 0.99

r = 0.98

ρ = 0.99

ρ = 0.99


r^{2} = 0.99

r^{2} = 0.96
  
Gabonese children (n = 28)
^{b}

MFI day 0

MFI day 84

PPFC day 0

PPFC day 84

kmeans

r = 0.71

r = 0.76

ρ = −0.93

ρ = −0.88


r^{2} = 0.51

r^{2} = 0.59
  
EM*

r = 0.61

r = 0.64

ρ = 0.78

ρ = 0.81


r^{2} = 0.38

r^{2} = 0.41
  
Overlap subtraction

r = 0.79

r = 0.83

ρ = 0.94

ρ = 0.96


r^{2} = 0.62

r^{2} = 0.69
  
 *Expectation Maximization. r = Pearson’s correlation coefficient. r^{2} = Coefficient of determination. ρ = Spearman correlation coefficient. Ab (IgG) responses are expressed as mean fluorescence intensity (MFI) and percentage of positive fluorescent cell (PPFC). Correlations for MFI were calculated using log_{10} transformed data. ^{a}Data excluded for 3 participants due to problems with data acquisition and inability of some algorithms to set an appropriate gate. ^{b}Two participants have been excluded from analysis for the same reasons as above. All pvalues are significant (p < 0.001) except for those marked with ^{§}.