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Table 2 Bland-Altman analyses of the different data gating strategies

From: A flow cytometry-based workflow for detection and quantification of anti-plasmodial antibodies in vaccinated and naturally exposed individuals

  Manual gating
Gabonese adults (n = 37)a MFI day 0 MFI day 84 PPFC day 0 PPFC day 84
k-means 611 600.9 −0.04 −0.06
  (464.3, 757.7) (350.5, 851.3) (−0.09, 0.005) (−0.12, -0.005)
Expectation −388.3 −328.7 0.02 0.01
Maximization (−600.2, -176.4) (−604.8, -52.7) (0.004, 0.04) (−0.003, 0.03)
Overlap subtraction −124.2 −98.1 0.006 −0.004
  (−160.3, -88.2) (−143.4, -52.7) (0.003, 0.009) (−0.02, 0.001)
Gabonese children (n = 28) b MFI day 0 MFI day 84 PPFC day 0 PPFC day 84
k-means 198.6 205.8 −0.4 −0.34
  (166.7, 230.6) (176.8, 234.8) (−0.49, -0.31) (−0.42, -0.27)
Expectation −208.9 −171.7 0.09 0.06
Maximization (−290.4, -127.3) (−214.5, -128.9) (0.03, 0.14) (0.05, 0.08)
Overlap subtraction 39.8 56.2 −0.04 −0.05
  (25.1, 54.5) (43.6, 68.9) (−0.03, -0.05) (−0.06, -0.03)
  1. Ab reactivity is expressed as mean fluorescence intensity (MFI) and percentage of positive fluorescent cell (PPFC). Data is given as mean differences of MFI and PPFC values (lower and upper 95% confidence interval) between the different approaches. aData excluded for 3 participants due to problems with data acquisition and inability of some algorithms to set an appropriate gate. bTwo participants have been excluded from analysis for the same reasons as above.