Table 2 Sequences of oligonucleotide primers and cycling conditions used for full-length pfatpase6 sequencing
Primer Sequence (5′→3′) | Ann (min) | Size (bp) |
|---|---|---|
F1: ATTATATCTTTGTCATTCGTG | 55 (1’) | 840 |
R1: TTGTAAAGGTGTTTGAGTATC | ||
F2: TCATCTACCGCTATTGTATG | 60 (1’) | 775 |
R2: TCCTCTTAGCACCACTCC | ||
F3: AAGTGTTGAGACGTTAGGATG | 55 (1’) | 698 |
R3: TTGATGATTGTACAGGTGTTG | ||
F4: TGGAGACAGTACCGAATTAGC | 60 (1’) | 814 |
R4: TCTTCCTACATATTTACGTGGTG | ||
F5: ATTGTAAAGGTGCACCTGAG | 60 (1’) | 922 |
R5: TTACCTAGTGCTGTTGCTGG | ||
F6: TAGTAATATAGGAGAAGTTGC | 60 (1’) | 578 |
R6: TGTATGTTTGTGTGTGTGC | ||
F7: ATCCACCAGAACATGACG | 60 (1’) | 760 |
R7: TCTTGGTTCTTTGCTCTTC |