Clustering, selection and classification of orthologous groups. (A) Selection of orthologous groups. Clustering of predicted proteins from five species (P. vivax, P. knowlesi, P. falciparum, P. berghei, P. yoelii) according to orthologs groups defined in OrthoMCL (version 4). Numbers in blue: orthologous groups; in red: total protein numbers. (B) Classification of groups according to signal peptide predictions based on the status of their proteins. The numbers in blue indicate orthologous groups. (C) Categorization of Mixed groups, after visual inspection, into three categories: (i) No misannotations; (ii) Containing putative misannotations; and (iii) Inconclusive. The Containing putative misannotations category was divided into two subcategories: (i) Reannotated; and (ii) Partially reannotated. Numbers in blue: orthologous groups, in pink: numbers of putative misannotated proteins into each category/subcategory. (D) Group reclassification of signal peptide prediction after protein reannotations. Numbers in blue: orthologous groups; in green inside square brackets: reannotated proteins; in orange inside curly brackets: putatively misannotated proteins that could not be revised. To the right, graphs representing the percentages of orthologous groups in each panel, the plotted labels are indicated by square boxes matching the colors in the graphs.