Experimental validation of proposed new gene models. Left panels show the amplification of Plasmodium vivax cDNA isolated from an infected patient. Right panels show schematic representations of the original gene model (light blue boxes) and the new model (light green boxes). PCRs were done using Control (light grey arrow), Before (dark blue arrow) and After (dark green arrow) forward primers with the same Reverse (black arrow) primer in the presence (+) or absence (-) of reverse transcriptase. The resulting amplicons (Before – blue line; Control – grey line; After – green line), with their respective molecular sizes, are shown in the middle of right panels for genes encoding for proteins (description according to BDA results): [PlasmoDB:PVX_081500] adenyl cyclase associated protein (A), [PlasmoDB:PVX_083205] protein transport protein Sec61 alpha subunit (B), [PlasmoDB:PVX_083025] sporozoite microneme protein (C), [PlasmoDB:PVX_002580] pseudouridine synthetase (D), [PlasmoDB:PVX_118150] glutamine cyclotransferase (E), [PlasmoDB:PVX_100770] conserved hypothetical protein (F) and [PlasmoDB:PVX_116975] conserved hypothetical protein (G). Dashed linear lines in gene models represent the 5' UTR of mRNAs. N – negative PCR control (without DNA); M - Molecular marker (1Kb Plus, Invitrogen). The schematic representations of the gene models were not in scale.