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Table 4 Specificity of anti-CSP repeats ELISA using sera pre-incubated with different antigens

From: Validation of an enzyme-linked immunosorbent assay for the quantification of human IgG directed against the repeat region of the circumsporozoite protein of the parasite Plasmodium falciparum

Sample Code Concentration of anti-CSP repeats antibody (EU/ml) Inhibited with: % inhibition
R32LR AMA-1 HBsAg CONTROL* R32LR AMA-1 HBsAg
1 37 70 69 76 51.3 7.9 9.2
2 80 134 141 138 42.0 2.9 −2.2
3 56 97 102 105 46.7 7.6 2.9
4 97 156 160 162 40.1 3.7 1.2
5 146 249 243 250 41.6 0.4 2.8
6 51 89 98 97 47.4 8.2 −1.0
7 84 148 167 167 49.7 11.4 0.0
8 124 213 223 217 42.9 1.8 −2.8
9 121 222 235 222 45.5 0.0 −5.9
Mean      45.2 4.9 0.5
  1. AMA-1, apical membrane antigen-1; CSP, circumsporozoite protein; HBsAg, hepatitis B surface antigen.
  2. * control: no inhibitory antigen used (co-incubation with diluent).