Table 1 Primer sequences for PCR amplification of the five molecular markers

Pvama-1

5′–CCAGCTGGAAGATGTCCTGT–3′ (F)

5′–TAATCCGAACTTGGCGTTTC–3′ (R)

Pvcsp

Primary

5′–ATGTAGATCTGTCCAAGGCCATAAA–3′ (F)

5′–TAATTGAATAATGCTAGGACTAACAATATG–3′ (R)

Secondary

5′–GCAGAACCAAAAAATCCACGTGAAAATAAG–3′ (F)

5′–CCAACGGTAGCTCTAACTTTATCTAGGTAT–3′ (R)

Pvmsp-1 (F2)

Primary

5′–GATGGAAAGCAACCGAAGAAGGGAAT–3′ (F)

5′–AGCTTGTACTTTCCATAGTGGTCCAG–3′ (R)

Secondary

5′–AAAATCGAGAGCATGATCGCCACTGAGAAG–3′ (F)

5′–AGCTTGTACTTTCCATAGTGGTCCAG–3′ (R)

Pfmsp-1

Primary

5′–CTAGAAGCTTTAGAAGATGCAGTATTG–3′ (F)

5′–CTTAAATAGATTCTAATTCAAGTGGATCA–3′ (R)

Secondary K1

5′–AAATGAAGAAGAAATTACTACAAAAGGTGC–3′ (F)

5′-GCTTGCATCAGCTGGAGGGCTTGCACCAGA–3′ (R)

Secondary MAD20

5′–AAATGAAGGAACAAGTGGAACAGCTGTTAC–3′ (F)

5′–ATCTGAAGGATTTGTACGTCTTGAATTACC–3′ (R)

Secondary RO33

5′–TAAAGGATGGAGCAAATACTCAAGTTGTTG–3′ (F)

5′–CAAGTAATTTTGAACTCTATGTTTTAAATCAGCGTA–3′ (R)

Pfmsp-2

Primary

5′–GAAGGTAATTAAAACATTGTC–3′ (F)

5′–GAGGGATGTTGCTGCTCCACAG–3′ (R)

Secondary FC27

5′–GCTTATAATATGAGTATAAGGAGAA–3′ (F)

5′–GCATTGCCAGAACTTGAA–3′ (R)

Secondary 3D7

5′–GCTTATAATATGAGTATAAGGAGAA –3′ (F)

5′–CTGAAGAGGTACTGGTAGA–3′ (R)