| |
Anopheles plumbeus
|
Anopheles gambiae Ngousso
|
---|
|
Day of observationa
|
Exp. 1
|
Exp. 2b
|
Exp. 3
|
Exp. 4
|
Exp. 1
|
Exp. 2
|
Exp. 3
|
Exp. 4
|
---|
Prevalence (%) of midgut infection (no. positive / no. analysed)
|
8
|
67
|
ndc
|
40
|
4
|
100
|
100
|
87
|
73
|
|
(20/30)
| |
(12/30)
|
(1/24)
|
(30/30)
|
(30/30)
|
(26/30)
|
(22/30)
|
15
|
75
|
15
|
43
|
10
|
100
|
100
|
67
|
70
|
|
(36/48)
|
(2/13)
|
(22/51)
|
(7/70)
|
(32/32)
|
(24/24)
|
(20/30)
|
(21/30)
|
Intensity of infection: Mean no. of oocysts / positive femaled (range)
|
8
|
8.9 ± 6.3
|
ndc
|
50.4 ± 56.0
|
12.0 ± 0
|
42.4 ± 28.2
|
71.5 ± 63.3
|
29.5 ± 23.5
|
13.7 ± 13.6
|
|
(2–25)
| |
(1–171)
|
(12)
|
(3–122)
|
(1–233)
|
(1–74)
|
(1–48)
|
15
|
5.3 ± 4.9
|
2.0 ± 0
|
28.8 ± 14.4
|
2.4 ± 2.1
|
22.4 ± 25.2
|
44.8 ± 36.4
|
26.4 ± 23.7
|
10.7 ± 10.1
|
|
(1–18)
|
(2)
|
(4–63)
|
(1–6)
|
(1–112)
|
(1–146)
|
(1–79)
|
(1–45)
|
Prevalence (%) of females with sporozoitese (no. positive / no. analysed)
|
15
|
83
|
31
|
88
|
57
|
97
|
88
|
67
|
70
|
|
(40/48)
|
(4/13)
|
(45/51)
|
(40/70)
|
(31/32)
|
(21/24)
|
(20/30)
|
(21/30)
|
Intensity of infection: Mean no. sporozoites / positive femalef (no. analysed)
|
15
|
1,620
|
ndc
|
108
|
ndc
|
11,178
|
25,623
|
15,755
|
950
|
|
(12)
| |
(15)
| |
(30)
|
(30)
|
(30)
|
(30)
|
- Experiments 1 to 4 were performed in June, July, September and November, 2010, respectively.
- a After exposure to P. falciparum gametocytes.
- b Low number of An. plumbeus mosquitoes due to high mortality during transport.
- c Not determined.
- d Positive females are females with at least 1 detectable oocyst.
- e PCR detection[40] of P. falciparum DNA isolated from head/thorax.
- f Sporozoite quantification was performed using the Ozaki method[41]; total number was normalized to the oocyst prevalence at day 8.