Features characteristic of the copper transport protein family are conserved in the putative Plasmodium copper transporters. A. Three transmembrane (1–3), an extracellular amino terminal (Nt) and intracellular carboxy terminal (Ct) domains, methionine and MxxxM and GxxxG motifs of copper transport proteins. B. An aligned region of the Plasmodium and other copper transporters showing the MxxxM and GxxxG motifs. Sequence identifiers are: Hs = Homo sapiens; Ms = Mus musculus; Tp = Theileria parva; Cp = Cryptosporidium parvum; Pf = P. falciparum PF14_0369; Pv = P. vivax; Py = P. yoelii; Pb = P. berghei; Pc = P. chabaudi; Pg = P. gallinaceum; Pk = P. knowlesi; Pr = P. reichenowi. Underlined amino acids (Hs) indicate the positions of transmembrane domains two and three. C. Cladogram comparing six complete Plasmodium copper transporter sequences with seven characterized transporters from various organisms. D. PCR amplification of the PF14_0369 coding domain from P. falciparum gDNA. Lane 1, full-length PF14_0369 coding domain; lane 2, amino terminal domain of PF14_0369; lane 3, P. falciparum LDH (PF13_0141) control. A Fermentas DNA ladder (M) is shown to the left of the gel.