Figure 5
From: A Plasmodium falciparum copper-binding membrane protein with copper transport motifs
Expression of the putative P. falciparum copper transporter (PF14_0369) in synchronized asexual parasites. Asexual P. falciparum parasites were grown in vitro, synchronized by sorbitol treatment and samples removed at five time points. Equivalent amounts of 1 x 107 synchronous, saponin-isolated P. falciparum parasites were analysed by A. 12.5% reducing SDS-PAGE, blotted onto nitrocellulose and probed with chicken anti-LDH peptide antibodies B. and anti-PF14_0369 peptide antibodies C. In all panels: lane 1 (L1), early rings; lane 2 (L2), late rings/early trophozoites; lane 3 (L3), trophozoites; lane 4 (L4), late trophozoites; lane 5 (L5), schizonts. Representative images of each stage of asexual development are shown below panel A (L1 – L5). M are the prestained molecular marker proteins.