Table 1 Primer sequence and polymerase chain reaction (PCR) program for MSP1 and MSP2 amplification
Gene | Name | Primer sequence | PCR program |
|---|---|---|---|
msp 1 primary | O1 | 5′ CACATGAAAGTTATCAAGAACTTGTC 3′ | 94°C-3 min, [94°C-25 sec, 50 °C-45 sec, 68°C-2 min] × 30, 72°C-3 min |
O2 | 5′ GTACGTCTAATTCATTTGCACG 3′ | ||
msp 1 nested | N1 | 5′ GCAGTATTGACAGGTTATGG 3′ | 94°C-3 min, [94°C-30 sec, 50 °C-45 sec, 68°C-2 min] × 30, 72°C-3 min |
N2 | 5′ GATTGAAAGGTATTTGAC 3′ | ||
msp2 primary | S3 | 5′ GAAGGTAATTAAAACATTGTC 3′ | 94°C-3 min, [94°C-30 sec, 42′C-60 sec, 65°C-2 min] × 30, 72°C-3 min |
S2 | 5′ GAGGGATGTTGCTGCTCCACAG 3′ | ||
msp2 nested | S1 | 5′ GAGTATAAGGAGAAGTATG 3′ | 94°C-3 min, [94°C-30 sec, 50 °C-60 sec, 72°C-2 min] × 45, 72°C-3 min |
S4 | 5′ CTAGAACCATGCATATGTCC 3′ |