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Figure 1 | Malaria Journal

Figure 1

From: An improved single-step lysis protocol to measure luciferase bioluminescence in Plasmodium falciparum

Figure 1

Comparison of performance of the standard and single-step lysis protocols. (A) The mean and stdev (n = 5) of the relative luminescent units (RLU) from 20 μl of packed IE subject to the indicated pellet-volumes of 1 × RLB is shown. The key indicates the protocol used for each assay. (B) Comparison of bioluminescent signal derived using single-step lysis protocol for the indicated buffer ± 0.1% saponin supplement. As a control (hatched bar), the same IE culture was subject to 1 × RLB and the standard lysis protocol.

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Malaria Journal

ISSN: 1475-2875

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