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Figure 2 | Malaria Journal

Figure 2

From: An improved single-step lysis protocol to measure luciferase bioluminescence in Plasmodium falciparum

Figure 2

Optimization of the single-lysis step protocol. (A) The mean ± stdev of RLU normalized for parasite number is compared to the pellet-volumes of PLB/0.1% saponin lysis buffer. (B) Comparison of the mean ± stdev RLU against total parasite number shows a strong linear correlation. Bioluminescence was measured from varying volumes of IE pellet supplemented with either PBS (filled circles) or uIE (unfilled circles) to a total volume of 20 μl.

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Malaria Journal

ISSN: 1475-2875

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