A rapid and scalable density gradient purification method for Plasmodium sporozoites

Malaria Journal

Table 1 Measurements of the reduction of bacterial contaminants by penicillin/streptomycin pre-treatment and Accudenz of P. yoelii sporozoites

	-	-	+	+	Accudenz Purification
	-	+	-	+	Penicillin/Streptomycin
Replicate 1	0.84	0.68	0.017 (98.0%)	0.0080 (99.0%)
Replicate 2	1.2	2.1	0.040 (96.6%)	0.0080 (99.3%)
Replicate 3	1.0	1.6	0.022 (97.8%)	0.012 (98.8%)

Salivary gland sporozoites were resuspended in RPMI 1640, left untreated or treated with penicillin/streptomycin, and were left unpurified or were purified on an Accudenz discontinuous gradient. Sporozoites were pelleted and washed in RPMI 1640, and then serially diluted and plated on LB-agar plates. Total colony forming units per sporozoite are listed for three biological replicates. Percent reductions of residual bacterial burdens for purified sporozoites compared to unpurified sporozoites not treated with penicillin/streptomycin are listed in parenthesis.

ISSN: 1475-2875