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Table 2 Measurements of the time to blood stage patency indicate that there is no loss of infectivity with purified P. yoelii sporozoites

From: A rapid and scalable density gradient purification method for Plasmodium sporozoites

Type Replicate # # Sporozoites injected # Mice infected # Mice patent (Day of patency)
Unpurified 1 10,000 5 5 (3d)
  2 10,000 5 5 (3d)
  1 1,000 5 5 (4d)
  2 1,000 5 5 (4d)
Purified 1 10,000 5 5 (3d)
  2 10,000 5 5 (3d)
  1 1,000 5 5 (4d)
  2 1,000 5 5 (4d)
  1. Salivary gland sporozoites were isolated from mosquitoes at 14d post-blood meal and injected intravenously into groups of five naïve Swiss Webster mice in duplicate. Time to blood stage patency (defined as >1 parasite per 20,000 RBCs) was determined microscopically by daily Giemsa-stained thin blood smears, and was identical for sporozoites pretreated with antibiotics or for those left untreated.