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Figure 1 | Malaria Journal

Figure 1

From: Simultaneous determination of phagocytosis of Plasmodium falciparum-parasitized and non-parasitized red blood cells by flow cytometry

Figure 1

Fluorescent labelling of np- and p-RBCs with CF-SE. (A) Dependence of MFI on CFDA-SE concentration (μM final) and haematocrit in np-RBC. (black circle) 0.05%, (black square) 0.5%, (black triangle) 5% haematocrit, corresponding to 5 x 103, 5 x 104, 5 x 105 RBCs/μl, respectively. (B) CF-SE fluorescence of np-RBCs (full grey histogram), ring-parasitized RBCs (light grey line), and troph-parasitized RBCs (dark grey line). np- and p-RBCs were incubated as indicated in (A). (C) Absence of fluorescence quenching in np-RBCs after phagocytosis. Fluorescence of CF-SE-labelled uningested np-RBCs (full grey histogram, MFI: 48.3 ± 1.6, N = 3) and of phTHP-1 cells fed with limiting numbers of CF-SE-labelled np-RBCs (RBCs/THP-1 ratio = 1, full black histogram, MFI: 49.7 ± 1.1, N = 3) were very similar. For details, see Methods.

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