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Figure 2 | Malaria Journal

Figure 2

From: Simultaneous determination of phagocytosis of Plasmodium falciparum-parasitized and non-parasitized red blood cells by flow cytometry

Figure 2

Phagocytosis by THP-1 cells of positive control np-RBCs opsonized with anti-D IgG or treated with zinc/BS3. (A) CF-SE fluorescence vs side scatter density plots showing THP-1 cells fed with non-opsonized untreated (left plot), anti-D IgG-opsonized (second plot), serum-opsonized, zinc/BS3-treated (third plot) and non-opsonized, zinc/BS3-treated (right plot) np-RBCs. Data are percentages of phagocytically active THP-1 cells above the fluorescence threshold set in each experiment. One representative experiment out of five with similar results. (B) Phagocytosis of variously treated positive control np-RBCs. Data are numbers of np-RBCs phagocytosed per phTHP-1 cell. Mean values ± SD (N = 5) ( *P < 0.001). For details, see Methods.

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