Figure 6

Expression of PF13_0006 on the surface of activated gametocytes (gametes). Activated gametocytes (emerging gametes) of the P. falciparum 3D7 line were fixed but not permeabilized and stained with anti-PF13_0006 IgG (green) and anti-Pfs230 Ab (red) (first two rows) and anti-PF13_0006 IgG (green) and anti-glycophorin A (red) (third row). As a control gametes were stained with control IgG (green) and anti-Pfs230 Ab (red). Nuclei were stained with DAPI (blue). DIC shadow-cast images with the fluorescence image superimposed in the first four coloumns and the fluorescence image (FI) alone in the last coloumn. Scale bar 5 μM.