Figure 3

The IgG response and prevalence to gSG6 and fSG6 according to the level of mosquito bites and Anopheles populations ( i.e., proportion of mosquito species). Box plots of aOD values from un-exposed (n = 45) and exposed (Diama, n = 46; Dielmo, n = 38 and Ndiop, n = 50) individuals to gSG6 (A) and fSG6 (B) proteins. Antibody responses are represented by aOD: the mean OD value of wells with recombinant salivary proteins minus the mean OD value of wells with coating buffer. The box plots display the median aOD value, 25th and 75th percentile. The whiskers indicate the 90th and 10th percentiles and the dots indicate the outliers. The P value was determined according to a Mann–Whitney U test (*, p <0.05; **, p <0.01; ***, p <0.001). The seroprevalence to gSG6 (C) and fSG6 (D) proteins in the four sites. The cut-off value for seropositivity (the mean aOD ± 3 standard deviations) was defined at 0.55 for gSG6 and 0.59 for fSG6, based on the IgG reactivity of sera from individuals living in Marseille that were not previously exposed to An. gambiae s.l. and An. funestus. Individuals showing aOD values above the cut-off level for seropositivity were classified as responders. The whiskers denote the 95% CI. The P values were determined by Pearson’s Chi-squared test (*, p <0.05; **, p <0.01; ***, p <0.001).