Figure 6
From: Anti-malarial activity of geldanamycin derivatives in mice infected with Plasmodium yoelii
Serum samples from geldanamycin treated mice have antibodies against vast majority of parasite proteins. Protein extracts from (A) P. yoelii and (B) mouse rbcs were analyzed on 12% SDS-PAGE. Gels were either silver stained (lanes 1, 6, 8 & 10) or blotted on PVDF membrane and probed with various serum samples collected from mice. (A) P. yoelii cell extract: silver stained (lane 1); Western using naïve mice serum (lane 2); malaria sensitive mouse serum (lane 3); serum from GA treated mice (lane 4) and serum from chloroquine treated mice (lane 5). (B) Mouse RBC extract: un-infected (lanes 6 & 7), ~3% parasitemia (lanes 8 & 9) and ~30% parasitemia (lanes 10 & 11). Lanes 6, 8 & 10 are silver stained while lanes 7, 9 & 11 are western blots developed using pooled sera from GA treated mice.