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Table 2 Programme used for pfdhfr amplification

From: Efficacy of artemether-lumefantrine in treatment of malaria among under-fives and prevalence of drug resistance markers in Igombe-Mwanza, north-western Tanzania

Name PCR programme
Outer 94°C 3 min; 5 cycles:94°C 30 sec, 56°C 30 sec, 72°C 45 sec; 8 cycles: 92°C 30 sec, 55°C 30 sec, 72°C 45 sec; 12 cycles: 92°C 30 sec, 53°C 30 sec, 72°C 45 sec; 18 cycles: 92°C 30 sec, 50°C 30 sec, 72°C 45 sec; 16°C hold
Nested
(pfdhfr3+pfdhfr4)
94°C 3 min; 30 cycles:94°C 1 min, 50°C 1 min, 72°C 1 min; 72°C 10 min; 4°C hold
Nested
(pfdhfr5+pfdhfr6)
94°C 3 min; 30 cycles: 94°C 1 min, 55°C 1 min, 72°C 1 min; 72°C 10 min; 4°C hold