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Figure 4 | Malaria Journal

Figure 4

From: Transcript and protein expression profile of PF11_0394, a Plasmodium falciparum protein expressed in salivary gland sporozoites

Figure 4

PF11_0394/GFP-trafficking studies demonstrate that the PF11_0394 protein is present in salivary gland sporozoites. The PF11_0394 protein is not detected during mixed erythrocytic stages (ES, culture representing a mixture of rings, trophozoites, and schizonts), mixed gametocytes (culture representing a mixture of stage I-V gametocytes), zygotes, ookinetes, oocyst sporozoites, and haemolymph sporozoites. The PF11_0394 protein is detected in salivary gland sporozoites. 3D7HT-GFP (3D7-GFP) constitutively expressing parasites were used as a positive control and wild-type (WT) parasites were used as a negative control [23]. This figure is a representative image from two biological replicates (one with each independent clone created) with two technical replicates each. Yellow arrowheads depict the presence of parasites that lack GFP expression (merged images). GFP = green fluorescent protein and nuclear stain = DAPI. The black scale bar in the lower right represents all of the images (except for the oocyst sporozoite stage) and is 10 μm. The yellow scale bar for the oocysts is 20 μm.

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