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Table 1 Sensitivity of RDT-DNA extraction methods in in vitro cultured parasites

From: Rapid diagnostic tests for molecular surveillance of Plasmodium falciparum malaria -assessment of DNA extraction methods and field applicability

RDT Fragment

Simple elution

Chelex-100

ABI

Paracheck-Pf

1 cm

§

2

200

Proximal

NA

20

200

Distal

NA

NA

20

Whole

NA

NA

20

SD-Bioline Malaria P.f/Pan

1 cm

2

2

20

Proximal

NA

2

20

Distal

NA

NA

20

Whole

NA

NA

20

Filter paper

5 μL blood spot

200

2

200

  1. Lowest achieved parasite detection levels (parasites/μL) for DNA extraction from Paracheck-Pf, SD-Bioline Malaria P.f/Pan and from 5 μL blood spotted on filter paper.
  2. § = Not estimated due to negative results.
  3. NA = Not applicable, the size of the RDT fragment used for extraction was limited by the extraction volume.