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Table 2 An outline for a possible TPP for a rapid test to detect G6PD deficiency used prior to the treatment of vivax malaria

From: Review of key knowledge gaps in glucose-6-phosphate dehydrogenase deficiency detection with regard to the safe clinical deployment of 8-aminoquinoline treatment regimens: a workshop report

Feature Ideal specification Comments
Output Level of G6PD deficiency In the absence of a consensus a quantitative read out may be most appropriate. In the absence of a consensus a qualitative read out may be controversial.
Use case On patients with confirmed malaria infection. This is an additional cost to malaria case management.
User Someone who performs malaria RDTs or microscopy. A quantitative read-out could come from a reader or scanner.
Sensitivity and Specificity Must accurately classify all patients with G6PD levels below a pre-defined cut-off. No patients with potentially dangerously low G6PD levels should be misclassified as normal. It is unclear on what the suitable cut-off should be. Too low and the risk of haemolysis increases. Too high primaquine is denied to more patients who need it.
Environmental tolerance 25-38°C, 40-90% humidity Enzyme activity is extremely temperature sensitive.
Result read window < 20 minutes Technically challenging for an enzyme reaction.
Specimen type Finger stick Currently very limited data with finger stick specimens.