Figure 2
Southern blot detection of transgenes integrated into the genomes of Anopheles stephensi driver and effector transgenic lines. Genomic DNA extracted from a pool of mosquitoes from each line was digested with SpeI, fractionated by agarose gel electrophoresis, transferred to a Zeta-probe GT membrane and hybridized to a 32P-labelled DsRed2 open-reading frame probe. DAa, DAs and E and the numbers on top of the figures indicate the individual transgenic lines originated from injection of the OX3545 and AsOX3534 driver and OX3547 effector constructs, respectively.