Table 1 Enzymes and control used, number and size of fragments obtained by codon
Restriction enzymes | Wild type alleles/control used | Number and size of bands obtained (bp) | Mutant alleles/control used | Number and size of bands obtained (bp) |
|---|---|---|---|---|
pfdhfr : Nested PCR product size : 594 | ||||
Alu I | S108/3D7 | 2bands : 280+ 314 | N108 or T108 HB3, Dd2 | 1 band : 594 |
Tsp 509 I | N51/3D7 FRC3 | 2 bands : 125+ 150 | I51/Dd2 | 2bands : 125+ 250. |
Xmn I | C59/3D7, FCR3 | 2bands : 250+ 344 | R59/Dd2 | 2bands : 250+ 320 |
pfdhps : Nested PCR product size : 711 | ||||
Ava II | A437/FCR3 | 1band : 711 | G437/3D7, Dd2 | 1band : 650 |
Fok I | K540/3D7, Dd2, FCR3 | 1band : 711 | E540/D10 | 2bands : 311 + 400pb |
pfcrt : Nested PCR product size : 145 | ||||
Apo I | K76/3D7, HB3 | 2 bands : 99 + 46 | T76/Dd2 | 1 band : 145 |
pfmdr1 : Nested PCR product size : 521 | ||||
AFI III | N86/3D7, HB3 | 1 band : 521 | Y86/FCR3 | 2 bands : 346 + 175 |