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Table 1 Enzymes and control used, number and size of fragments obtained by codon

From: Prevalence of the molecular marker of Plasmodium falciparum resistance to chloroquine and sulphadoxine/pyrimethamine in Benin seven years after the change of malaria treatment policy

Restriction enzymes Wild type alleles/control used Number and size of bands obtained (bp) Mutant alleles/control used Number and size of bands obtained (bp)
pfdhfr : Nested PCR product size : 594
Alu I S108/3D7 2bands : 280+ 314 N108 or T108 HB3, Dd2 1 band : 594
Tsp 509 I N51/3D7 FRC3 2 bands : 125+ 150 I51/Dd2 2bands : 125+ 250.
Xmn I C59/3D7, FCR3 2bands : 250+ 344 R59/Dd2 2bands : 250+ 320
pfdhps : Nested PCR product size : 711
Ava II A437/FCR3 1band : 711 G437/3D7, Dd2 1band : 650
Fok I K540/3D7, Dd2, FCR3 1band : 711 E540/D10 2bands : 311 + 400pb
pfcrt : Nested PCR product size : 145
Apo I K76/3D7, HB3 2 bands : 99 + 46 T76/Dd2 1 band : 145
pfmdr1 : Nested PCR product size : 521
AFI III N86/3D7, HB3 1 band : 521 Y86/FCR3 2 bands : 346 + 175