Pvarp gene transcription during blood stage. Lane 1 indicates the molecular weight marker. Lanes 2–4 show Pvarp gene amplification using cDNA RT+, RT- and gDNA, respectively. Lanes 6–8 show the amplification of the Pvron1-a region using cDNA RT+, RT- and gDNA as template, respectively. Lane 5 shows the negative control for amplifying the Pvarp gene.