Figure 2
Analyses by TLC of products synthesized by rPfFPPS. The activity of rPfFPPS was measured by a 14C radioactivity assay, utilizing [4-14C]IPP and DMAPP, GPP, or FPP as allylic substrate. The enzymatic reactions (Protocol I) and TLC were performed as described in the Methods section. Lane 1, [4-14C]IPP + DMAPP as substrates; lane 3, [4-14C]IPP + GPP as substrates; lane 5, [4-14C]IPP + FPP as substrates; lanes 2, 4 and 6 control reactions without enzymes for reactions showed on lanes 1, 3 and 5 respectively. Products labelled with [4-14C]IPP were visualized by a Bioscan System 200 Imaging Scanner. The positions of geraniol (GOH), farnesol (FOH) and geranylgeraniol (GGOH) standards are indicated on the left. The main products detected were FOH and GGOH, indicating that this were the major enzyme products. Ori: origin SF: front.