Identification of minimal human MHC-restricted CD8+ T-cell epitopes within the Plasmodium falciparum circumsporozoite protein (CSP)

Table 1 Volunteer HLA A and B allele groups and supertypes

Vaccine	Vol.	Strategy			Trial	HLA-A1 allele group	HLA-A2 allele group	HLA-B1 allele group	HLA-B2 allele group	HLA-A1 supertype	HLA-A2 supertype	HLA-B1 supertype	HLA-B2 supertype
		1A	1B	2
Ad-CA	v1	x			1	A*02:01	A*26:01	B*18:01	B*44:02	A02	A01	B44	B44
	v2	x			1	A*01:01	A*02:01	B*08:01	B*44:02	A01	A02	B08	B44
	v5	x			1	A*01:01	A*68:02	B*08:01	B*14:02	A01	A02	B08	B27
	v8	x			1	A*68:01	A*68:02	B*14:02	B*48:01	A03	A02	B27	B27
	v12	x			1	A*30:02	A*68:01	B*18:01	B*58:02	A01	A03	B44	B58
Ad-CA	v125			x	2	A*02:01	A*11:01	B*35:01	B*52:01	A02	A03	B07	B62
	v127		x		2	A*01:01	A*24:02	B*08:01	B*44:05	A01	A24	B08	B44
	v156		x	x	2	A*03:01	A*29:02	B*15:03	B*58:02	A03	A01 A24	B27	B58
Ad-C	v37		x	x	3	A*23:01	A*68:02	B*15:03	B*53:01	A24	A02	B27	B07
	v40		x	x	3	A*23:01	A*29:02	B*52:01	B*53:01	A24	A01	B62	B07
	v41	x			3	A*02:01	A*31:01	B*07:02	B*35:01	A02	A03	B07	B07
	v49	x			3	A*33:01	A*74:01	B*15:03	B*15:03	A03	A03	B27	B27
	v58	x	x		3	A*02:01	A*24:02	B*08:01	B*38:02	A02	A24	B08	B27¹
	v61	x			3	A*02:01	A*02:01	B*38:01	B*44:02	A02	A02	B27	B44
	v63			x	3	A*11:01	A*24:03	B*40:01	B*51:04	A03	A24	B44	B07
	v68			x	3	A*24:02	A*30:01	B*13:02	B*14:02	A24	A01 A03	B62¹	B27
	v69	x	x		3	A*30:02	A*34:02	B*14:02	B*35:01	A01	A03	B27	B07

The volunteers from whom PBMC were available and were tested are shown. Trial 1 (no CHMI): five of six volunteers immunized with Ad-CA were used (reference 7). Trial 2 (with CHMI): three of 17 volunteers immunized with Ad-CA and challenged by bite of P. falciparum-infected mosquitoes were used (Tamminga, in press). Trial 3 (with CHMI): nine of 11 volunteers immunized with Ad-C and challenged by bite of P. falciparum-infected mosquitoes were used (reference [8]). The table also identifies the ten volunteers with best available PBMC that were included in the broad screen of all 15-mer peptides from the four dominant pools (column 1A), the six volunteers used to confirm recognition of minimal epitopes predicted within positive 15-mers (column 1B) (1A+1B=strategy 1), and the six volunteers used to confirm recognition of minimal epitopes predicted within 15-mers without the initial screen (strategy 2).
¹As classified in reference [42].

ISSN: 1475-2875