Identification of minimal human MHC-restricted CD8+ T-cell epitopes within the Plasmodium falciparum circumsporozoite protein (CSP)

Table 3 ELISpot IFN-γ activity of CSP peptide pools and individual 15-mer peptides within these pools

Pool	Vol.	Vaccine	15mer peptide	AA no.	Sequence	Pool sfc/m	15mer sfc/m	Percent of pool
Cp1	58	Ad-C	C3	9-23	SVSSFLFVEALFQEY	116	29	25.0
Cp1	05	Ad-CA	C3		SVSSFLFVEALFQEY	142	65	45.8
Cp1	69	Ad-C	C3		SVSSFLFVEALFQEY	411	385	93.7
Cp1	12	Ad-CA	C4	13-27	FLFVEALFQEYQCYG	64	87	135
Cp1	05	Ad-CA	C4		FLFVEALFQEYQCYG	142	44	31.0
Cp1	01	Ad-CA	C4		FLFVEALFQEYQCYG	77	48	62.3
Cp1	69	Ad-C	C4		FLFVEALFQEYQCYG	411	368	89.5
Cp1	58	Ad-C	C5	17-31	EALFQEYQCYGSSSN	116	105	90.5
Cp1	58	Ad-C	C6	21-35	QEYQCYGSSSNTRVL	116	83	71.6
Cp2	49	Ad-C	C8	29-43	SSNTRVLNELNYDNA	64	41	64.1
Cp2	41	Ad-C	C10	37-51	ELNYDNAGTNLYNEL	83	35	42.2
Cp2	49	Ad-C	C12	45-59	TNLYNELEMNYYGKQ	64	28	43.8
Cp2	12	Ad-CA	C12		TNLYNELEMNYYGKQ	331	411	124
Cp2	01	Ad-CA	C12		TNLYNELEMNYYGKQ	119	119	100
Cp2	01	Ad-CA	C13	49-63	NELEMNYYGKQENWY	119	116	97.5
Cp2	12	Ad-CA	C13		NELEMNYYGKQENWY	331	334	100
Cp6	61	Ad-C	C47	313-327	DKHIKEYLNKIQNSL	53	48	90.6
Cp6	41	Ad-C	C48	317-331	KEYLNKIQNSLSTEW	95	103	108
Cp6	05	Ad-CA	C48		KEYLNKIQNSLSTEW	130	106	81.5
Cp6	58	Ad-C	C48		KEYLNKIQNSLSTEW	24	45	187
Cp9	61	Ad-C	C60	365-379	EKKICKMEKCSSVFN	128	39	30.5
Cp9	01	Ad-CA	C62	373-387	KCSSVFNVVNSSIGL	39	116	297
Cp9	08	Ad-CA	C63	377-391	VFNVVNSSIGLIMVL	142	109	76.8
Cp9	08	Ad-CA	C64	381-395	VNSSIGLIMVLSFLF	142	91	64.1
Cp9	01	Ad-CA	C65	383-397	SSIGLIMVLSFLFLN	39	46	117
Cp9	02	Ad-CA	C65		SSIGLIMVLSFLFLN	16	44	275
Cp9	08	Ad-CA	C65		SSIGLIMVLSFLFLN	142	62	43.7

All individual 15mer peptides within the CSP peptide pools Cp1, Cp2, Cp6 and Cp9 were tested in ELISpot assay using frozen PBMC collected 28 days after Ad-CA or 19-23 days after Ad-C immunization. 15 individual 15mer peptides of the 26 15mer peptides elicited positive recall responses from at least one volunteer immunized with Ad-CA and Ad-C, and each of the ten volunteers responded positively to at least one 15mer.

ISSN: 1475-2875