Identification of minimal human MHC-restricted CD8+ T-cell epitopes within the Plasmodium falciparum circumsporozoite protein (CSP)

Table 6 ELISpot IFN-γ activity of CSP predicted epitopes after depletion of CD4+ and CD8+ T cells compared with ICS CD8+ and CD4+ T cell IFN-γ activity

Vol.	Pool	Epitope no.	Sequence	HLA allele group	Control depl. sfc/m	CD8+ depl. sfc/m (%)^*	CD4+ depl. sfc/m (%)^*	CD8+ %^**	CD4+ %^**
V40¹	Cp1				97	5 (-95%)	82 (-15%)	0.44	0.02
		E21	SFLFVEALF	A*23:01	254	15 (-94%)	226 (-11%)	0.37	0.04
		E14	SVFNVVNSSI	A*23:01	86	38 (-56%)	43 (-50%)	0.12	0.01
		E18	LIMVLSFLF	A*23:01	236	23 (-90%)	210 (-11%)	0.54	0.02
V69²	Cp1				445	6 (-98%)	397 (-11%)	0.53	0.01
V69²		E2	FVEALFQEY	B*35:01	380	17 (-96%)	356 (-6%)	0.48	0.00
V125³		E20	SVSSFLFV EA	A*02:01	79	72 (-9%)	17 (-78%)	0.06	0.02
V156³	Cp1				133	0 (-100%)	135 (+2%)	0.33	0.01
		E21	SFLFVEALF	A*29:02	213	29 (-86%)	273 (+28%)	0.25	0.01
		E1	FLFVEALFQ E	A*29:02	245	91 (-73%)	215 (-12%)	0.34	0.03
	Cp9	E18	LIMVLSFLF	A*29:02	307	85 (-82%)	264 (-18%)	0.26	0.01

¹PBMC from one month after the first Ad-C immunization; ²PBMC from 19 days after the second Ad-C immunization; ³PBMC from 28 days after challenge following Ad-CA immunization.
*The percent change in ELISpot activity is shown after depletion of CD4+ or CD8+ T cells.
**The percent of CD8+ or CD4+ gated T cells expressing IFN-γ.
depl. = depletion.
sfc/m = spot forming cells/million.
v125 did not have sufficient PBMC to test Cp1.

ISSN: 1475-2875