Figure 4

IC ₅₀ determination using JC-1 and/or pLDH in parallel on drug treated P. falciparum cultures. Culture P. falciparum-infected erythrocyte were incubated for 48 h in the presence of the indicated drug concentration at haematocrit of 5% and 1% parasitaemia. Panel A - shows that the inter-experiment variability between 2 separate JC-1 determinations is low (red and violet lines) and that the methodology is more accurate (IC₅₀ is consistently lower) than the IC₅₀ determination obtained with the pLDH methodology; Panel B - shows typical curves obtained by splitting parasites derived from a same mother culture into plates, in which they were cultured in parallel under the same environmental conditions in the presence of rising artemisinin concentrations and IC₅₀ curves where either determined using the pLDH (violet line) or the JC-1 (blue line) methodology; Panel C – shows typical curves obtained by splitting parasites derived from a same mother culture into plates, in which they were cultured in parallel under the same environmental conditions in the presence of rising atovaquone concentrations and IC₅₀ curves where either determined using the pLDH (violet line) or the JC-1 (blue line) methodology.