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Table 1 Comparison of qPCR and multiplex PCR techniques for detection of Plasmodium falciparum sporozoites in salivary glands of Anopheles gambiae

From: Evaluation of a real-time quantitative PCR to measure the wild Plasmodium falciparum infectivity rate in salivary glands of Anopheles gambiae

Salivary glands samples

qPCR positive

qPCR negative

Total

Sensitivity

Specificity

Kappa (κ)

PCR positive

217

0

217 (60%)

100%

60%

0.63

PCR negative

59

88

147 (40%)

   

Total

276 (76%)

88 (24%)

364 (100%)

   
  1. McNemar test: qPCR vs multiplex PCR, P <0.001.