SYBR Green I fluorescence linearity in Plasmodium falciparum ring stages of 0-10% parasitaemia. Data points show average RFUs of 12 replicate wells, derived from one experiment using the P. falciparum K1 clone, plotted on the Y axis against % parasitaemia on the X axis. Red and black symbols, lines, and letters represent samples spiked with 8,500 WBC/μL and samples without WBCs, respectively. Regression line and R2 are shown. Dashed lines indicate LOD and LOQ range parasitaemia values obtained from up to three experiments.