Figure 2From: Molecular epidemiology of Plasmodium vivax in Latin America: polymorphism and evolutionary relationships of the circumsporozoite geneMolecular size variation and RFLP patterns of the csp gene of Plasmodium vivax isolates from the central Pacific Ocean coast region of the Americas A and B, PCR amplified product size. A) Mexican samples; ~700 pb -vk210a (lane 1) and ~700 pb -vk210b (lane 2) had the same molecular size. Lane 3 and 4, show mixed genotype infections: (vk210b and ~500 pb- vk210c) and (>700 pb-vk247 and vk210c), respectively. B) Nicaraguan and Mexican samples; fragments of ~700 bp (lanes 4, 5 and 8; Nir1), ~650 bp (lanes 1, 3 and 9; Nichn), ~600 bp (lanes 2 and 7) and vk210a from Mexico (lane 6) are shown.RFLP patterns: C) Mexican samples; vk210d (lane 1–2), vk210b ( Lane 3–4), vk210c +vk247 (lane 5–6), and vk247+vk210a (lane 7–8). D) Mexican and Peruvian samples; vk210a (lanes 1–4, two samples), vk247 (lanes 5–8, two samples: Mexican and Peruvian); and mixed infection Vk210a-vk247 (lane 9–10). E) Nicaraguan samples; vk210a like - Nir1 isolate (lane 1 and 2), vk210e - Nichn isolate (lane 3–4), vk210f - Nir2 isolate (lane 5 and 6) are shown. C, D and E: odd numbers indicate Alu1 digestion and round numbers indicate BstI digestion. 1% agarose gels and ethidium bromide for staining were used. M, marker 1 kb.Back to article page